Our Instruments
Instruments available to our users are
About the FACS Aria 2
- Enables purification of cell populations for downstream assays
- Enclosed in a biosafety cabinet for safe sorting ofBSL-2 materials
- Three lasers, up to nine channels
- Sorting into tubes or 96-well plates
- Operated by CAC personnel (Andy Sebrell)
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Laser
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Filters
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Fluorochromes
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|---|---|---|
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488 nm
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530 nm
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FITC
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576 nm
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PE
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616 nm |
PE-Tx Red
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695 nm |
PerCP-Cy5.5, PI
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780 nm
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PE-Cy7
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561 nm
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582 nm
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Tomato Red
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633 nm
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660 nm
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APC
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780 nm
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APC Cy7
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About the BioRad ZE5™
- 5 lasers, up to 30 parameters
- User-friendly plate and universal tube loader
- Small particle detector for bacteria, viruses, and exosomes
- Temperature control and orbital agitation
- Fast data collection – up to 100,000 cells/second
- Please acknowledge USDA 2023-70410-41180
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5 Lasers (27 Colors)
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Laser
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Filter
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Fluorochrome
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355
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387/11
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BUV395
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447/60
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AF350, DAPI
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525/50
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BUV496
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670/30
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BUV611
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700LP
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BUV737
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405
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420/10
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BV421
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460/22
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SBV440, Pacific Blue
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525/50
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SBV515, BV510, CFP
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615/24
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SBV610, BV605
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670/60
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SBV670, BV650
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720/60
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BV711
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750LP
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BV786
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488
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488/10
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SSC
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525/35
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FITC, GFP, YFP, AF488, BB515
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593/52
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PE, PE-CF594,
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692/80
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SBB700, PerCp-Cy5.5, PE-Cy5
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750LP
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BB790, PE-AF750, PE-Cy7
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561
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577/15
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PE
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589/15
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DsRed, td Tomato
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615/24
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PE-Dazzle, PE-CF594, mCherry
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640/20
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mPlum
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670/30
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PE-Cy5, PE-AF647
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720/60
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PE-Cy5.5
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750LP
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PE-Cy7, PE-AF750
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640
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670/30
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APC, AF647
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720/60
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AF700
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775/50
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APC-Cy7, AF750
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800LP
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AF790, APC/Fire810
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About the Stratedigm SE520EON
- Located in Cooley Lab
- Two lasers, up to six colors and eight parameters (incl. FSC/SSC)
- Acquired with support from INBRE (P20GM103474)
| Laser | Filers | Fluorochromes |
|---|---|---|
| 448 nm | 530 nm | FITC |
| 580 nm | PE | |
| 676 nm | perCP / PE-Cy5 | |
| 780 nm | PE-Cy7 | |
| 640 nm | 676 nm | APC |
| 780 nm | APC Cy7 |
About the FACS Calibur
- Located in Cooley Lab
- Older instrument
- Two lasers, six parameters
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Laser
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Filters
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Fluorochromes
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|---|---|---|
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488 nm
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530 nm
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FITC, GFP, AF488
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585 nm
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PE, PI
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670 nm
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7-AAD, PerCP, PerCP-Cy5.5, PE-Cy5, PE-Cy7
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635 nm
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661 nm
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APC, AF647
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About the BD LSR Fortessa
- Located in Cooley Lab
- High-end BD cell analyzer
- 18 colors, 20 total parameters
- Available for self-service to trained users
- Diva software
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Laser
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Filters
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Fluorochromes
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|---|---|---|
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355 nm (UV laser) |
450 nm
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DAPI
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535 nm
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Indo-1
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405 nm
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450 nm
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BV421, Pacific Blue, DAPI
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535 nm
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BV510, V500, Pac. Orange
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610 nm
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QDot605
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670 nm
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BV650, Qdot 655
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710 nm
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BV711, Qdot 705
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488 nm
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530 nm
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FITC, Alexa Fluor 488,
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695 nm
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PerCP-Cy5.5
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561 nm
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582 nm
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PE
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610 nm
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PE-Tx Red
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660 nm
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PE-CY5
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710 nm
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PE-CY5.5
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780 nm
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PE-CY7
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640 nm
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670 nm
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APC
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780 nm
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АРС СУ7
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790 nm
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AF700
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About the ImageStream Mark II
- High throughput analyses
- Equipped with 20x, 40x and 60x objectives
- Can perform image analysis on >10,000 events
- Records >100 cellular features
- IDEAS software has integrated machine learning algorithms
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Channel |
Band
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Excitation laser (nm)
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|---|---|---|---|---|
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488
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642
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785
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1
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435-505
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2
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505-560
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FITC, AF488,GFP, YFP, Dylight488, PKH67, Syto13, LysoTrackerGreen, MitoTrackerGreen
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3
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560-595
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PE, PKH26, DSRed, mOrange, Sytox Orange, Cy3
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4
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595-642
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PE-TexRed, PI, RFP, QD625, eFluor625
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5
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642-745
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PECy5, PEAF647, 7AAD, PerCP, PerCp-Cy5.5, DRAQ5, QD705
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APC, AF647, AF660, AF680, APC, Cy5, Dylight649, PE-AF647, PE-Cy5, DRAQ5, PerCP, PerCP-Cy5.5
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6
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745-780
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PE-Cy7, PE-AF750, QD800
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APC-Cy7, APC-AF750, APC-H7, Cy7, AF750, PE-Cy7, PE-AF750
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SSC
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About the 10x Genomics ChromiumX
- Microfluidic system
- Processes cell suspensions for single cell sequencing
- The mRNA of each cell is tagged with a cell-specific nucleic acid barcode
- Users then generate cDNA and sequencing libraries
- Sequencing enables analysis of each individual cell’s complete transcriptome
